Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken anti-GFP | Aves Labs | Cat# GFP-1010; RRID: AB_2307313 |
| Mouse anti-Apocrypta bakeri Orco | Laboratory of Vanessa Ruta Butterwick et al. 76 |
15B2 |
| Goat anti-mouse Cy5 | Invitrogen | Cat# A10524; RRID: AB_2534033 |
| Goat anti-chicken Alexa Fluor 488 | Invitrogen | Cat# A11039; RRID: AB_2534096 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Critical commercial assays | ||
| HCR RNA in situ probes, amplifiers, buffers; see DataS1 for additional details | Molecular Instruments | https://www.molecularinstruments.com/ |
| Deposited data | ||
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Anopheles gambiae: Wild-type G3 strain | Laboratory of Flaminia Catteruccia Greppi et al.11 |
N/A |
| Anopheles coluzzii: QUAS-mCD8:GFP strain | Laboratory of Christopher Potter Riabinina et al.45 |
N/A |
| Anopheles coluzzii: QUAS-GCAMP6f strain | Laboratory of Christopher Potter Afify et al.47 |
N/A |
| Anopheles gambiae: Ir93apore-T2A-Qf2-3xP3-RFP strain | This paper | N/A |
| Anopheles gambiae: Ir93apore-T2A-Qf2-3xP3-eYFP strain | This paper | N/A |
| Anopheles gambiae: Ir93aTM2-T2A-Qf2-3xP3-RFP strain | This paper | N/A |
| Anopheles gambiae: Ir93aTM2-T2A-Qf2-3xP3-eYFP strain | This paper | N/A |
| Anopheles gambiae: Ir21aeYFP strain | Garrity lab Greppi et al.11 |
N/A |
| Anopheles gambiae: Ir21a+7bp strain | Garrity lab Greppi et al.11 |
N/A |
| Aedes aegypti: Wild-type Liverpool (LVP) strain | Laboratory of Leslie Vosshall Matthews et al.71 |
N/A |
| Aedes aegypti: QUAS-mCD8:GFP strain | Laboratory of Carolyn McBride Matthews et al.37 |
N/A |
| Aedes aegypti: QUAS-GCAMP7s-T2A-tdTomato strain | Laboratory of Carolyn McBride Zhao et al.48 |
N/A |
| Aedes aegypti: Ir93a-T2A-Qf2-3xP3-RFP strain | This paper | N/A |
| Aedes aegypti: Ir93a-T2A-Qf2-3xP3-eYFP strain | This paper | N/A |
| Oligonucleotides | ||
| See DataS1 for assembled list of oligonucleotide sequences | This paper | N/A |
| Recombinant DNA | ||
| Plasmid: AgIr93apore-T2A-Qf2-3xP3-RFP HDR | This paper | N/A |
| Plasmid: AgIr93apore-T2A-Qf2-3xP3-eYFP HDR | This paper | N/A |
| Plasmid: AgIr93aTM2-T2A-Qf2-3xP3-RFP HDR | This paper | N/A |
| Plasmid: AgIr93aTM2-T2A-Qf2-3xP3-eYFP HDR | This paper | N/A |
| Plasmid: AaIr93a-T2A-Qf2-3xP3-RFP HDR | This paper | N/A |
| Plasmid: AaIr93a-T2A-Qf2-3xP3-eYFP HDR | This paper | N/A |
| Plasmid: AgU6C-Ir93a-pore-gRNA-AgVasa-Cas9 | This paper | N/A |
| Plasmid: AgU6C-Ir93a-TM2-gRNA-AgVasa-Cas9 | This paper | N/A |
| Plasmid: AaU6C-Ir93a-gRNA-AaExu-Cas9 | This paper | N/A |
| Software and algorithms | ||
| Fiji (2.3.0/1.5.3f) | ImageJ |
https://imagej.net/software/fiji/ RRID:SCR_002285 |
| Photoshop (21.2.2) | Adobe |
https://www.adobe.com/ RRID:SCR_014199 |
| Illustrator (24.2.3) | Adobe |
https://www.adobe.com/ RRID:SCR_010279 |
| JMP 11 | SAS |
https://www.jmp.com/ RRID:SCR_014242 |
| LabChart Pro V7 | AD Instruments |
https://www.adinstruments.com/ RRID:SCR_017551 |
| GraphPad Prism 9 | GraphPad |
https://www.graphpad.com/ RRID:SCR_002798 |
| Stackreg | Thevenaz et al.79 | http://bigwww.epfl.ch/thevenaz/stackreg/ |
| Other | ||