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. Author manuscript; available in PMC: 2024 Mar 17.
Published in final edited form as: Circ Res. 2023 Feb 17;132(6):723–740. doi: 10.1161/CIRCRESAHA.122.321604

Figure 2. Male mice have normal cardiac function, no changes in the number of LV and RV myocytes, and only RV cardiomyocytes have increased EdU labeling after hypoxia.

Figure 2.

A: Ejection fraction (EF), cardiac output relative to body weight (CO/BW), and E/E’ were measured with Echocardiography at baseline and during terminal studies (n=9-15). B: Cardiomyocytes were isolated from the LV and RV of these hearts and the total number of cardiomyocytes were quantified. C: Mouse hearts were stained for alpha-sarcomeric actin (αSA, red, label cardiomyocytes), EdU (green, DNA synthesis), and DAPI (blue, nuclei) to identify myocytes and nonmyocytes undergoing DNA synthesis. Total EdU+ cardiomyocytes and total EdU+ cells were quantified in LV and RV tissue sections (n=6-10). Data are shown as mean±SD.