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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Hepatology. 2023 Jan 3;77(4):1198–1210. doi: 10.1097/HEP.0000000000000016

Figure 6. Both Notch and Yap signaling are required for hepatocyte-to-BEC conversion.

Figure 6.

Confocal projection images showing the expression of Tp1:VenusPEST and Alcama in regenerating livers. (A, B) The Tg(hs:canotch3) line was used to enhance Notch signaling with a single heat-shock at BR0h; verteporfin (A) and the Tg(hs:dnyap1) line (B) were used to suppress Yap signaling. (C) The Tg(hs:cayap1) line was used to enhance Yap signaling with a single heat-shock at BR0h; LY411575 (1 μM) was treated from BR0h onwards to suppress Notch signaling. (D) Suboptimal doses of LY411575 (50 nM) and verteporfin (500 nM) were treated from BR0h onwards. (E-G) Hepatocyte-to-BEC conversion in fbxw7 heterozygous mutants. Given that Fbxw7 negatively regulates Yap signaling as well as Notch signaling, verteporfin (1 μM) was cotreated with LY411575 until BR0h, which permits one to determine the role of Fbxw7 in hepatocyte-to-BEC conversion. (H) A diagram depicting the epigenetic relationship between Notch and Yap signaling in hepatocyte-to-BEC conversion. The number of BECs per liver area is quantified and presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001; statistical significance was calculated using an unpaired two-tailed t-test (E) or one-way ANOVA (A-D,F,G). Scale bars: 50 μm.