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. 2023 Feb 9;30(3):383–390. doi: 10.1038/s41594-022-00913-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Predicted phenotypes of CA assembly behavior in vitro and CLP shape, as determined by the configuration of the TVGG motif. All but T = 1 icosahedra have been observed previously. b–e, Morphologies of CLPs formed by the indicated CA constructs in 25 mM MES, pH 6, 150 mM NaCl, 5 mM β-mercaptoethanol, and 5 mM IP6. Scale bars, 200 nm. f, 2D class averages (upper) and cryo-EM structure (lower) of T = 1 icosahedra made by CA-G60A G61P. The map is colored according to local resolution.