Extended Data Fig. 1. In vitro HIV-1 CA assemblies.
(a-c) Cryoimages WT HIV-1 CA CLPs assembled under the indicated conditions, representative of at least two independent experiments with different protein preparations. Reactions were incubated at 37 °C for 1 h. Scale bars, 100 nm. (d-k) Negative stain images of CLPs formed under the indicated conditions, representative of 2 to 3 independent experiments with different protein preparations. Scale bars, 200 nm. (l) The longest dimension of individual CLPs were quantified for each of the indicated CA assemblies in 5 mM IP6, pH 6. According to one study, the average length of native fullerene cones is around 109 nm [ref. 57]. Aggregate data from all collected images are presented as the mean ± SD of the indicated n measurements.