Table 1.

Data collection, processing and refinement statistics

	WT declination		WT declination + CPSF6-FG	T = 1 (CA-G60A G61P)	T = 1 (CA-M66A)	T = 1 (CA-G60A G61P M66A)
EMDB	EMD-26715		EMD-28186	EMD-28054	EMD-28057	EMD-26718
PDB	7URN		8EJL	8EEP	8EET	7URT
Data collection, processing and map calculation
Magnification	×81,000		×81,000	×81,000	×92,000	×81,000
Voltage (kV)	300		300	300	200	300
Electron exposure (e–/Å2)	50		54	50	48	50
Defocus range (μm)	0.5 to 2.5		0.75 to 2.5	0.5 to 2.5	0.5 to 2.5	0.5 to 2.5
Pixel size (Å)	1.08		1.08	1.08	0.75	1.08
Symmetry imposed	C5		C5	I	I	I
Particles	525,219		166,463	494,057	116,627	509,666
Map resolution (Å)	3.4		3.9	2.2	3.1	2.4
FSC threshold	0.143		0.143	0.143	0.143	0.143
Map resolution range (Å)	2.3–8.3		2.5–8.9	2.2–4.9	2.1–7.2	2.4–5.2
Coordinate modeling and refinement
	Chain A (pentamer)	Chains L,M,N (half-hexamer)
Initial model	PDB 4XFX	PDB 4XFX	PDB 7URN	PDB 7URT	PDB 7URT	PDB 4XFX
Model resolution (Å)	3.4	3.6	4.1	2.5	3.4	2.5
FSC threshold	0.5	0.5	0.5	0.5	0.5	0.5
Map sharpening B-factor (Å2)	146.9	146.9	159.3	97.0	144.4	109.7
Model composition
Non-hydrogen atoms	1,725	5,175	4,431	1,726	1,726	1,726
Protein residues	221	663	619	221	221	221
IP6	2	0	0	2	2	2
B-factors (Å2)	73.2	78.8	117.0	42.9	42.6
Root mean squared deviations
Bond lengths (Å)	0.006	0.004	0.005	0.002	0.004	0.004
Bond angles (°)	0.623	0.508	0.545	0.647	1.019	0.554
Validation
MolProbity score	1.57	1.42	1.81	1.33	1.37	1.34
Clash score	8.40	5.79	7.23	3.95	6.23	4.05
Poor rotamers (%)	0.43	1.24	3.04	1.59	1.07	1.60
Ramachandran plot
Favored (%)	97.26	97.87	97.82	100	99.54	99.09
Outliers (%)	0	0	0	0	0	0
Z-score	1.00	1.79	1.74	1.98	1.87	1.22