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. 2023 Feb 13;30(3):321–329. doi: 10.1038/s41594-023-00922-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — (a) Representative Micrograph and obtained 2D classes used for template picking. (b) Fourier shell correlation of P116 reports a final resolution of 3.3 Å according to the 0.143 cut-off criteria. (c) Local resolution map at different thresholds of the cryoEM density map (C1 symmetry) of P116 ranging from 2.5 to 5 Å. (d) Schematic processing overview for the P116 construct. The processing for P116 empty, P116 refilled and P116 + HDL was carried out in a similar manner.

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