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. 2023 Feb 13;30(3):321–329. doi: 10.1038/s41594-023-00922-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — CryoEM classes display different flexibility modes. Individual reconstructions are shown on the left column. Their variability is shown in the middle column. Plotting of the individual eigenvector weights shows, that no clear distribution is visible and a smooth transition between the individual vibrational modes. Here ‘eigenvector 3’ (EV3) is plotted against ‘eigenvector 4’ (EV4) that display the largest resemblance to the radius change and ‘eigenvector 4’ (EV4) is plotted against ‘eigenvector 5’ (EV5) that display the largest resemblance to the twist.