Table 2.
% of [3H]cholesterol transferred/mL | nmol, cholesterol transferred/mL/hour | nmol cholesterol transferred/mg P116a | |
---|---|---|---|
HDL to empty P116 | |||
Free cholesterol | 13.12 | 13.52 | 59.49 (6.3) |
Esterified cholesterol | 6.98 | 7.22 | 31.75 (3.3) |
HDL to original P116 | |||
Free cholesterol | 7.89 | 7.42 | 32.63 (3.4) |
Esterified cholesterol | 6.32 | 6.01 | 26.44 (2.8) |
aNumbers in parentheses are the estimated number of cholesterol molecules transferred per P116 subunit (assuming a molecular weight of ~105 kDa for the construct).