Fig. 5. Inhibition of LH^LepR neurons decreases consummatory behaviours.

a Schematic of optogenetic inhibition (left, middle) and image of NpHR expression in LH^LepR neurons (right). The experiment was repeated 8 times independently with similar results. 3V, the 3rd ventricle; STN, subthalamic nucleus; cp, cerebral peduncle. b Schematic of the consummatory behaviour test 5 and schedule of laser stimulation. c Raster plot of consummatory behaviours during (b) (n = 8 mice). d Average duration of consummatory behaviours (top). Calibrated graph (bottom) of the top. Two-sided paired t-test; **p = 0.006 (time bin 2–4 min vs 4–6 min), ***p = 0.0007 (time bin 4–6 min vs 6–8 min), ***p = 0.0009 (time bin 10–12 min vs 12–14 min), *p = 0.02 (time bin 12−14 min vs 14–16 min), *p = 0.019 (time bin 14–16 min vs 16–18 min). e–g Total duration (e), bout duration (f), and number (g) of consummatory behaviours. Two-sided paired t-test; ****p  < 0.0001 (e), ***p = 0.0009 (f), n.s., p = 0.93 (g). Data are mean ± s.e.m. See Supplementary Table 1 for statistics. Source data are provided as a Source data file. The schematics in a left and b were created using BioRender.