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. 2023 Mar 17;13:4433. doi: 10.1038/s41598-023-31227-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental set-up for comparison of tear fluid pre-processing methods, and protein distribution on a Schirmer strip using PEA technology by Olink Proteomics. Tear fluid from 5 healthy volunteers was collected with Schirmer strips. Strips were cut vertically into two equal portions. Out of one part of the strip, four 1.2 mm punches were taken from various locations of the strip (A: head; B: 0–10 mm; C: 10–20 mm; D: 20–30 mm). The other part of the strip was cut into horizontal locations (A: head; B: 0–10 mm; C: 10–20 mm; D: 20–30 mm) and subsequently the locations were eluted in 60 µL buffer. The punches as well as 1 out of 40 µl of the eluates were supplied to Olink Proteomics in a 96 wells plate.