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. 2023 Mar 17;13:4433. doi: 10.1038/s41598-023-31227-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Principal component analysis of the protein composition (92 proteins) of different locations of the strips using the punch versus elution method. Scatterplot of the first two principal components based on Z-score scaled protein composition of all samples (n = 20*2) measured by the Olink Target 96 Inflammation panel. Location A (head of the strip) groups differently than Schirmer strip locations B, C and D, most noticeably the punch method location A groups separately. The samples with bold symbols provided a warning on the Olink proteomics quality control, which reflects the possible interference of proteins in these samples with the internal control.