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. 2023 Mar 17;6:280. doi: 10.1038/s42003-023-04643-2

Fig. 4. RNA-Sequencing reveals roles for DIAPH1 in regulation of hepatic lipid metabolism.

Fig. 4

Ldlr −/− and Ldlr−/− Diaph1−/− male mice were fed WD for 16 weeks. a Hierarchical clustering of differentially expressed genes in liver tissue from the indicated N = 4 independent mice/group. b The expression of the indicated genes identified as differentially expressed in the RNAseq data between Ldlr −/− mice vs. Ldlr−/− Diaph1−/− mice was determined by RT-qPCR. c The expression of the indicated genes identified as not differentially expressed in the RNAseq between Ldlr −/− mice vs. Ldlr−/− Diaph1−/− mice was confirmed by RT-qPCR. The number of independent mice/group is indicated in the figure as individual data points. Statistical analyses regarding testing for the normality of data followed by appropriate statistical analyses were described in Materials and Methods. P-values (in b, c) were determined by unpaired T test or Wilcoxon rank-sum test depending on if the data passed the Shapiro-Wilk normality test.