Table 8.
Primers used for PCR.
| Target gene | Primer sequences (5′ → 3′) | Length (bp) | Reference |
|---|---|---|---|
| Capsular type (primer) | |||
| K1 (magA) | F: GGTGCTCTTTACATCATTGC | 1283 | Fang et al.56 |
| R: GCAATGGCCATTTGCGTTAG | |||
| K2 (K2wzy) | F: GACCCGATATTCATACTTGACAGAG | 641 | Turton et al.57 |
| R: CCTGAAGTAAAATCGTAAATAGATGGC | |||
| K5 (K5wzx) | F: TGGTAGTGATGCTCGCGA | 280 | Turton et al.57 |
| R: CCTGAACCCACCCCAATC | |||
| K54 (wzxK54) | F: CATTAGCTCAGTGGTTGGCT | 881 | Fang et al.58 |
| R:GCTTGACAAACACCATAGCAG | |||
| K57 (wzyK57) | F: CTCAGGGCTAGAAGTGTCAT | 1037 | Fang et al.58 |
| R: CACTAACCCAGAAAGTCGAG | |||
| K20 (wzyK20) | F: CGGTGCTACAGTGCATCATT | 741 | Fang et al.58 |
| R: GTTATACGATGCTCAGTCGC | |||
| mrkD | F: CCACCAACTATTCCCTCGAA | 240 | El Fertas-Aissani et al.30 |
| R: ATGGAACCCACATCGACATT | |||
| wcaG | F: GGTTGGKTCAGCAATCGTA | 169 | Turton et al.52 |
| R: ACTATTCCGCCAACTTTTGC | |||
| rmpA | F: ACTGGGCTACCTCTGCTTCA | 516 | Nadasy et al.59 |
| R: CTTGCATGAGCCATCTTTCA | |||
| fimH | F: TGCTGCTGGGCTGGTCGATG | 688 | Yu et al.60 |
| R: GGGAGGGTGACGGTGACATC | |||
| entB | F: ATTTCCTCAACTTCTGGGGC | 371 | El Fertas-Aissani et al.30 |
| R: AGCATCGGTGGCGGTGGTCA | |||
| irp-1 | F: TGAATCGCGGGTGTCTTATGC | 238 | El Fertas-Aissani et al.30 |
| R: TCCCTCAATAAAGCCCACGCT | |||
| iroD | F: GCATAGGCGGATACGAACAT | 531 | Luo et al.61 |
| R: CACAGGGCAATTGCTTACCT | |||
| iroN | F: CTGTCGGCATCGGTTTTATT | 556 | Yu et al.60 |
| R: TGGCGTGTCGATTATTACCA | |||
| clbA | F: ATGAGGATTGATATATTAATTGGAC | 735 | Lai et al.46 |
| R: ATTCTGCCCATTTGACGAATG | |||
| clbB | F: GATTTGGATACTGGCGATAACCG | 732 | Lai et al.46 |
| R: CCATTTCCCGTTTGAGCACAC | |||
| kfu | F: ATAGTAGGCGAGCACCGAGA | 520 | Yu et al.60 |
| R: AGAACCTTCCTCGCTGAACA | |||
| K. pneumoniae 16S-23S ITS* | Pf: ATTTGAAGAGGTTGCAAACGAT | 130 | Liu et al.53 |
| Pr1: TTCACTCTGAAGTTTTCTTGTGTTC | |||
*Internal transcribed spacer.