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. 2023 Mar 17;13:4463. doi: 10.1038/s41598-023-31712-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of CDDP and ferroptosis inhibitor Vitamin E on ovarian function and fertility in rats. (A, C) HE staining of the ovarian tissue structure in each group and the statistical results of the number of follicles in different developmental stages. (B, D) Masson trichrome staining of the ovarian tissue in each group observed under a microscope. (E, F, G) Serum FSH, E2 and AMH levels in each group. (H) Gross observation of uterine litter size of rats in each group. (I) Summary of embryo numbers at implantation sites of each group. The score of stained Masson trichrome Staining was quantitated using ImageJ software. Data are expressed as the means ± SEM. ns, * versus control, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, ns indicates no statistical significance. N, # versus CDDP + VE, # P < 0.05, ## P < 0.01, ###P < 0.001 and #### P < 0.0001, N indicates no statistical significance.