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. 2023 Mar 17;14:1506. doi: 10.1038/s41467-023-37208-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Domain organizations of the IFT-A components. IFT144, IFT140, IFT122_N, IFT122_C, IFT121, IFT139 and IFT43 are colored goldenrod, salmon, green, green yellow, turquoise, blue and violet, respectively. Black asterisks indicate the helices inserted between the second and third TPR repeats in IFT144, IFT140, IFT122, and IFT121. The head and base module components have light yellow and pale blue background colors, respectively. Models of IFT144_WD1, IFT144_C, IFT140_C and IFT139_N built with the aid of AlphaFold-2 are shown in dashed ovals or circles. The middle disordered loop of IFT43 is shown in a dashed line. b Silver staining SDS-PAGE gel (left) and native-PAGE gel (right) showing the purity and major conformations of the purified IFT-A complex. Each experiment was repeated three times independently with similar results. c A representative negative staining image of the IFT-A complex. Two different conformations of the particles are marked with red rectangle and circle, respectively. d 2D class averages of the negative staining images of the IFT-A complex. Cryo-EM density maps of the elongated (e) and folded (f) IFT-A complex in two orthogonal views. The head and base modules are colored in light yellow and pale blue, respectively. g A schematic diagram showing the conformational change of the head module from the elongated to the folded state. Source data are provided as a Source Data file.