Figure 4.

Genetic subgroups of BL are associated with unique transcriptomic patterns. (A) The heat map displays the 71 differentially expressed genes between subgroups, with rows representing differentially expressed genes and columns representing samples. Rows and columns are clustered on the basis of Pearson correlation. The top annotations indicate subgroup membership, EBV status, age, and sex. Although the separation is incomplete, when clustered on these genes, most DGG-BL cases cluster to the left, whereas most IC-BL cases cluster to the right. (B) Variance stabilized expression of IRF4 and TNFRSF13B, the genes with the strongest differential expression between DGG-BL and IC-BL. Expression values are along the y axis, with subgroup membership indicated along the x axis. Expression values are stratified on the basis of subgroup membership, with IC-BL exhibiting significantly elevated expression of both IRF4 and TNFRSF13B (∗∗∗∗P < .001; Wilcoxon rank sum test). (C) Heat map representing the hierarchical clustering of gene sets obtained from the signatureDB database. Samples are clustered and ordered on their expression of genes within each gene set. Rows represent the gene sets, and columns represent samples. Rows and columns are clustered on the basis of euclidean distance measure.