Figure 4.

Distinct RUNX1A and RUNX1C protein interaction networks. (A) Experimental setup for isolating HA-RUNX1A– or HA-RUNX1C–containing protein complexes from CMK cells. (B) RUNX1A and RUNX1C complex composition in CMK cells with functional grouping. The Venn diagram shows significantly enriched interactors (log₂ fold change > 1; P < .05). (C) Western blot confirming coimmunoprecipitation of MAX and RUNX1A using anti-MAX and anti-RUNX1 antibodies. Representative picture of 2 independent experiments using K562 cells are shown. A 10% input was used as the loading control. (D) Western blot showing RUNX1A/C isoforms coimmunoprecipitated with doxycycline-inducible HA-tagged GATA1 and GATA1s or the EV vector. Representative pictures of 3 independent experiments using CMK cells are shown. A 5% input was used as the loading control. IP, immunoprecipitation; mRNA, messenger RNA.