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. 2023 Mar 17;14:1417. doi: 10.1038/s41467-023-37101-y

Fig. 4. Expression of intact St6galnac1 restores Sialyl-Tn expression in conjunctival goblet cell mucins in vivo.

Fig. 4

a Schematic representation of backcrossing of B6J-derived intact St6galnac1 gene to Balb/c mice. b Chromosomal distribution of PCR-confirmed strain-specific markers in Ao mice. c Intact St6galnac1 expression in the conjunctiva. n = 7 for WT and n = 14 for Ao mice. ****p < 0.0001 by two-tailed Mann–Whitney test. Error bar represents S.E.M. d AB-PAS staining of the conjunctiva. Bar, 20 μm. e Immunostaining of sialyl-Tn in conjunctiva with or without neuraminidase treatment. WGA, wheat germ agglutinin Bar, 20 μm. f A schematic diagram of secreted mucus collection. Western blotting of the secreted mucus with (h, i) or without (g, i) neuraminidase treatment. The photographs of pollen shell amount left in the wells of polyacrylamide gels after the electrophoresis are also shown as loading controls (g–i). Data are representative of at least two independent experiments (d, e, g–i). Source data are provided as a Source Data file (c, g, h, i).