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. 2023 Mar 17;14:1417. doi: 10.1038/s41467-023-37101-y

Fig. 8. St6galnac1 protects mice against allergic conjunctivitis.

Fig. 8

a Clinical appearance of PBS- or ragweed pollen (RW)-challenged eyes. b Clinical scores. n = 2, 1, 7, and 7 for PBS-WT, PBS-Ao, RW-WT, and RW-Ao conditions, respectively (b, c, f). **p < 0.01 (p = 0.0093) by two-tailed Mann–Whitney test. c Scratch bouts. *p < 0.05 (p = 0.0449) by two-tailed Mann–Whitney test. d Giemsa staining of the conjunctiva. Arrowheads indicate eosinophils. Bar, 50 μm. Inset: Bar, 10 μm. e Eosinophil frequency among CD45 + cells in the conjunctiva. Eosinophil frequencies in live single cells after challenges of 100 mg/mL (f) or indicated amount (mg/mL) (g) of RW pollen or PBS. *p < 0.05 (p = 0.0379) by two-tailed Mann–Whitney test (f). **p < 0.01 (adjusted p = 0.0091, WT vs Ao in RW 50 mg/mL condition), ***p < 0.001 (adjusted p = 0.0006, WT vs Ao in RW 100 mg/mL condition) by two-tailed ANOVA with Holm-Sidak’s multiple comparisons test (g). h RW scores represent the amount of remaining pollen in the conjunctival sac 30 min after pollen challenges. n = 14 for each strain. *p < 0.05 (p = 0.0325) by two-tailed Mann–Whitney test. i, j Accumulation of migratory dendritic cells (CD11c+MHC class II high) to the draining lymph nodes 12 h after instillation of pollen shells and RW extract labeled with AF647. FACS gating for pooled data (i) and enumeration (j) are shown. N, PBS-instilled; S/RWe, pollen shell and AF647-labeled RW extract. PBS-instilled data were used for setting AF647 gate and therefore excluded from statistical analysis. n = 4, 7, 4, and 8 for WT-N, WT-S/RWe, Ao-N, and Ao-S/RWe conditions, respectively. **p < 0.01 (p = 0.0037) by two-tailed Mann–Whitney test. Data are representative of four (a–h) or three (i, j) independent experiments. Error bar represents S.E.M. (b, c, f, g, h, j) Source data are provided as a Source Data file (b, c, f, g, h, j).