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. 2023 Mar 11;4(1):102145. doi: 10.1016/j.xpro.2023.102145

Washing buffer for X-Gal staining

Reagent Final concentration Amount
Deoxycholic acid, 10% 0.01% 50 μL
NP-40, 10% 0.02% 100 μL
1× PBS 49.85 mL
Total 50 mL

Store at 4°C for 6 months.