Preparation of primary antibody
| Reagent | Final concentration | Amount |
|---|---|---|
| Anti-HCN4 guinea pig antibody (1:200) | 1:200 | 2 μL |
| Anti-Nppa rabbit antibody (1:200) | 1:200 | 2 μL |
| 1× Universal blocking buffer | – | 396 μL |
| Total | – | 400 μL |
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