FIG. 6.

Features of the ORF-1 protein. (A) Hydropathy profile of ORF-1. The hydropathy value was determined by the method of Kyte and Doolittle (95) with a window of 9 aa. Hydrophobic regions are indicated by positive values. Depicted above the hydrophobicity profile are the wild-type ORF-1 and ORF-1-TTL₁₇₂ mutant peptides and their tumorigenicity. Adapted from reference 87 with permission of the publisher. (B) Alignment of ORF-1 protein amino acid sequences with domains 2 and 3 of members of the HCMV US22 family of proteins. The schematic shows regions of alignment (solid rectangles) that were detected as statistically significant (P ≤ 0.003) by the MACAW software (153).