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FRESH manufacturing procedure. (a) Gelatin slurry portioned into a 50-mL conical tube before centrifugation. (b) Post-centrifuge showing water and ethanol supernatant on top and deep-yellow colored compacted gelatin gel at the bottom of the conical tube. (c) Compacted gelatin gel in tube after decanting supernatant. (d) FRESH microsphere bath used for bioprinting after three rounds of centrifugation, washing, and decantation. FRESH, freeform reversible embedding of suspended hydrogel.
