FIG. 4.
Printing resolution and construct fidelity evaluation using the commercial FRESH LifeSupport and the optimized FRESH. (a) Illustration of measurement locations on the single filament construct for printing resolution analysis. Scale bar = 1 mm. (b) The Stanford “S” construct designed for fidelity testing. The preset dimensions were 10 mm in height, 6.2 mm in width, and 1.6 mm in middle thickness for bioprinting. (c) Bar graph comparison of single filament bioprinting results using the commercial FRESH and optimized FRESH. Optimized FRESH single filament has significantly reduced diameter compared with the commercial FRESH single filament. (d) Bar graph comparison of “S” construct bioprinting results using the commercial FRESH and optimized FRESH. “S” constructs printed using the commercial FRESH and optimized FRESH have similar dimensions with no significant difference. Error bars represent standard deviation. *p < 0.05.