Figure 3. Spatial organization of cells in the mouse frontal cortex and striatum across ages.
(A) (Left) spatial segmentation of anatomical regions. (Right) spatial organization of major cell types at the three different ages, colored by cluster identity. Dashed lines outlining anatomical regions were manually traced from spatial segmentation. Scale bar: 500 μm.
(B) Fraction of cells resided in individual anatomical regions for each cell cluster at the three different ages. CC: corpus callosum. Olf: subcortical olfactory areas. The lower abundance of ependymal cells in younger animals may be due to classification as molecularly similar astrocytes or loss of ventricle surface during tissue sectioning.
(C–F) Spatial organizations of oligodendrocyte (C), astrocyte (D), microglial (E), and endothelial (F) clusters at different ages. Scale bar: 500 μm.