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. 2023 Mar 18;13:4488. doi: 10.1038/s41598-023-31684-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Rarefaction analysis, calculated at 97% dissimilarity, for the assessment of operational taxonomic unit (OTU) coverage within the16S rRNA gene–based bacterial communities in the gastrointestinal tract of the four emus (Dromaius novaehollandiae) sampled in this study. (A) The number of OTUs as a function of the number of sequence reads. (B) The number of OTUs as a function of the number of individual emu sampled.