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. 2023 Mar 18;13:4488. doi: 10.1038/s41598-023-31684-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Venn diagram showing (A) the distribution of all 343 duodenum OTUs, calculated at 97% dissimilarity, identified in the 52,880 16S rDNA sequences. 13 OTUs were common to all 4 individuals. (B) The distribution of all 219 Jejunum OTUs identified in 61,139 sequences. 2 OTUs were common to the 4 individuals. (C) The distribution of all 438 ileum OTUs identified in 51,567 sequences. 14 OTUs were common to the 4 individuals. (D) The distribution of 129 OTUs common in all 4 individual in the different intestinal segments. Only 1 OUT was found in all 4 segments. The cecum data were obtained from Bennett et al.¹³ (D1–D4: duodenum of the 4 emus examined, J1–J4: jejunum, I1–I4: ileum, C1–C4: ceca).