Fig. 5.

ARID1A regulates HCC malignant behaviour by modulating RBL1 expression.

(a, b) GO function and KEGG pathway enrichment analysis was performed for genes significantly associated with ARID1A. (c) Western blot analysis confirmed the effects of ARID1A OE and KD on the JNK/FOXO3 signalling pathways in HCC. (d–f) The correlation between ARID1A, FOXO3 and RBL1 mRNA expression was analysed from the TCGA database. (g) The expression of ARID1A and RBL1 in HCC tissues was assessed by IHC staining of a human HCC TMA. (h) Statistical analysis showed that ARID1A expression was significantly correlated with RBL1 expression in HCC. (i, j) Changes in RBL1 mRNA levels and protein levels after knockdown or overexpression of ARID1A. (k) ChIP‒qPCR revealed that FOXO3 binds to the RBL1 promoter. (l) Schematic diagram of the mechanism by which ARID1A regulates HCC progression through the JNK/FOXO3/RBL1 axis. Unadjusted Western blot images are available in the Supplementary Material a, b.