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. 2023 Mar 4;62:102657. doi: 10.1016/j.redox.2023.102657

Fig. 2.

Fig. 2

Measurement of H2S and NO levels in BAEC during hyperglycemia. (A) The concentration of H2S was detected in the supernatant of BAEC exposed to the HG environment (3 h). *p < 0.05 vs NG. (B) Levels of H2S in the total cell lysate of BAEC exposed to HG environment. *p < 0.05 vs NG. (C) The concentration of total NO levels (expressed as total nitrate/nitrite, NOx) in BAEC exposed to HG environment and in presence of an increasing concentration of H2S donor AP123 (1–100 nM, 2 h). ***p < 0.001 vs NG; #p < 0.05, ###p < 0.001 * vs HG. (D) Live fluorometric quantification of H2S produced in BAEC exposed to NG, HG and HG with H2S donor AP123 (10 nM). *p < 0.05 vs NG; #p < 0.05 vs HG. The graphs show the mean values ± SEM for each group (n = 3–5). Differences have been considered statistically significant when p was ≤0.05.