Fig. 4.

Measurement of H₂S, eNOS and CREB protein expression levels in BAEC pre-treated with a CSE inhibitor in presence of AP123. (A) Fluorimetric quantification of live H₂S concentration produced in BAEC pre-treated with the CSE inhibitor, PAG (10 mM, 15 min) and stimulated with AP123 (10 nM). (B) Western blot analysis showing cropped images of the expression of eNOS and pCREB/CREB in AP123-stimulated BAEC pre-treated or not with PAG. Densitometric analysis of (C) eNOS and (D) pCREB/CREB ratio. The graphs represent the mean ± SEM for each group (n = 3–6). CTR: untreated cells; V: cells treated with PAG only; AP123: cells treated with PAG + AP123 10 nM. The β-actin has been used as a housekeeping control protein. *p < 0.05 vs. NG or CTRL; ^#p < 0.05 vs. vehicle + PAG. Differences have been considered statistically significant when p was ≤0.05.