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. 2023 Mar 8;62:102661. doi: 10.1016/j.redox.2023.102661

Fig. 2.

Fig. 2

Cholesterol directly regulates maintenance and lineage differentiation of HSCs in vivo. (A) Serum LDL-C levels in mice with control diet (CD) or HCD (n = 6). (B) Flow cytometric analysis of intracellular cholesterol contents in BM HSPCs of CD and HCD mice (n = 6). (C) Relative LDLR expression on BM LT-HSCs of CD and HCD mice (n = 6). (D) Flow cytometric analysis of BM LT-HSC pool size in CD and HCD mice (n = 6). (E) Flow cytometric analysis of frequency of LT-HSC subpopulations in CD and HCD mice (n = 6). (F) Frequencies of T cells, B cells, and myeloid cells in PB and BM of CD and HCD mice (n = 6). (G) Flow cytometric analysis of frequencies of CMPs, CLPs, GMPs, and MEPs in BM of CD and HCD mice (n = 6). (H) Schematic of competitive BM transplantation. (I) Fraction of donor-derived T cells, B cells, and myeloid cells in PB of recipient mice (n = 6). (J) Frequency of donor-derived cells in PB of recipient mice (n = 6). Data are mean ± SD. n.s., not significant. *p < 0.05, **p < 0.01. Two-tailed unpaired Student’s t-test.