Fig. 5.

Involvement of miR-34a and miR-17–5p in mediating MALAT-1 induced effects of innate and adaptive immune arms.

a) To verify that MALAT-1 mediates its effects on NK cell-mediated cytotoxic activity through miR-34a, MDA-MB-231 cells were co-transfected with both MALAT-1 siRNAs and miR-34a antagomirs was prepared. This abolished NK cytotoxic activity in comparison to MDA-MB-231 cells treated with MALAT-1 siRNAs or miR-34a mimics. b) To verify the involvement of miR-17–5p in mediating MALAT-1 effects on CD8+ T cells, MDA-MB-231 cells were co-transfected with MALAT-1 siRNAs and miR-17–5p antagomirs, this resulted in a significant reduction of the cytotoxic effects of CD8+ T cells compared with MDA-MB-231 cells treated with MALAT-1 siRNAs or miR-17–5p mimics.