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. 2023 Feb 17;299(4):103043. doi: 10.1016/j.jbc.2023.103043

Figure 8.

Figure 8

Glycolysis in macrophages following inflammatory stimuli is primarily mediated by p38 MAPK. WT and Mkp-1−/− BMDM were plated into a 24-well plate. The next day, cells were fed with fresh medium treated with DMSO, 10 μM SB203580, 3 μM JNK-IN-8, or 10 μM SB203580 plus 3 μM JNK-IN-8 for 30 min and then stimulated with 100 ng/ml LPS or heat-killed Escherichia coli (MOI: 10:1) for 16 h or left unstimulated. Medium was harvested and lactate levels in the media were measured using a fluorometric lactate assay kit. ∗p < 0.05, compared to DMSO-pretreated samples of the same genotype under the same stimulation; #, p < 0.05, compared to WT samples of the same stimulation and pretreatment. ¥, <0.05, compared to samples of the same genotype pretreated with SB203580 and of same treatment (t test, n = 4). MAPK, mitogen-activated protein kinase; MKP, MAPK phosphatase; BMDM, bone marrow–derived macrophage; LPS, lipopolysaccharide.