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. Author manuscript; available in PMC: 2023 May 7.
Published in final edited form as: Neuroimage. 2022 Sep 20;263:119640. doi: 10.1016/j.neuroimage.2022.119640

Figure 3: LFP recordings reveal activations in M1 for all stimulations of neurons specific to M1 layers, and in CPu and VL for stimulations within L4, L5, and L6 only, which correlates with fMRI activations.

Figure 3:

(A) Schematic shows recording optrode and electrode locations in ipsilateral M1, CPu, and VL. (B) Average LFP (n = 4 animals per Cre-line, total N = 16 animals) from ipsilateral M1, VL, and CPu neuron-specific M1 stimulation at 5 Hz shows robust activation in M1 for all Cre-lines, and in CPu and VL for Scnn1a (within L4), Rbp4 (within L5) and Ntsr1 (within L6) Cre-lines. (C) Change in LFP amplitude during stimulation was calculated to quantify the LFP traces. Rbp4 (within L5) stimulation evoked the strongest change in LFP amplitude in ipsilateral M1. Drd3 (within L2/3) stimulation evoked the weakest change in LFP amplitude in ipsilateral CPu. For ipsilateral VL, Ntsr1 (within L6) stimulation evoked the strongest change in LFP amplitude, while Rbp4 (within L5) and Scnn1a (within L4) stimulation evoked stronger activations compared to Drd3 (within L2/3). These LFP results are similar to the fMRI results. One-way ANOVA followed by Bonferroni’s post hoc test was used (*p < 0.05, **p < 0.01 and ***p < 0.001; error bars indicate ±SEM). (D) Scatter plot shows correlation between change in LFP amplitude and BOLD-fMRI area under the curve in ipsilateral M1, CPu, and VL for all Cre-lines (p < 0.001).