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. 2023 Feb 6;3(3):100261. doi: 10.1016/j.xgen.2023.100261

Figure 5.

Figure 5

Automated longitudinal imaging of dopaminergic neurons

(A) Time-lapse imaging of dopaminergic neurons (PPMI4110) expressing synapsin-I-driven GFP. Analysis started on day 55–56 of differentiation. One neuron (green arrowhead) survives the entire duration of imaging. A second neuron (red arrow) dies at 96 h. Scale bar: 60 μm.

(B) Cumulative risk-of-death curves showing the neuronal survival from all batch-1 lines over 8 days of automated imaging.

(C) Cumulative risk-of-death curves show increased degeneration in dopaminergic neurons differentiated from GBA1 PD lines compared with healthy control lines over 8 days of automated imaging (∗∗∗∗p < 0.0001; based on 891 neurons from GBA1 lines and 647 neurons from healthy control [HC] volunteers).