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. 2023 Feb 20;24(2):bbad050. doi: 10.1093/bib/bbad050

Figure 3.

Figure 3

metaFlye (with and without Pilon polishing) generated the most contiguous plasmid assemblies. Circular depictions of E. coli contigs in the single 150 kb plasmid present in the I3 isolate, assembled from spike-ins into the B1 background. Areas of correct assembly (blue) and misassemblies (red), as determined by metaQUAST, are shown for increasing spike-in coverages, ranging from 0x (innermost band) to 50x (outermost blue/red band). The outermost yellow and purple band indicates locations of ARGs (purple) and transposons (yellow) in the isolate genome. Assemblies are shown for (A) MEGAHIT (SR), (B) metaSPAdes (SR), (C) OPERA-MS (HY-SL), (D) metaFlye with Pilon polishing (HY-LS), (E) metaFlye (LR). Similar trends were seen across all 15 plasmids (SI Figure S2).