Skip to main content
. 2023 Mar 6;10:1111108. doi: 10.3389/fmolb.2023.1111108

FIGURE 3.

FIGURE 3

Platelets of Panx1 deficient mice reveal reduced platelet degranulation, activation and PS exposure during the remodeling phase of AAA development in the murine PPE model. Washed platelets from PPE operated Panx1 WT (Panx1 fl/fl -Pf4-Cre − ) and Panx1 KO (Panx1 fl/fl -Pf4-Cre + ) mice were analyzed via flow cytometry at day 14 (A), and at day 28 (B) after surgery. (A,B) Platelet degranulation (P-selectin-FITC), integrin αIIbβ3 activation (JON/A-PE) and PS-exposure (Annexin V-Cy5 binding) were analyzed after platelet stimulation with indicated agonists. In addition, platelet surface expression of GPVI, integrin α5, integrin β3 and GPIbα was analyzed via flow cytometry (PANX1 WT n = 6–11; PANX1 KO n = 4–6). Data are represented as mean values ±SEM. Statistical analysis was performed using an (A,B) unpaired multiple student’s t-test; *p < 0.05, **p < 0.01. ADP, Adenosine diphosphate; CRP, Collagen-related peptide; MFI, Mean fluorescence intensity; Panx1, Pannexin-1; PAR4, Protease-activated receptor 4 peptide; PPE, Porcine pancreatic elastase perfusion; Rest, resting; Thr, Thrombin; U46619 (U46), Thromboxane A2 analogue.