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. 2023 Mar 6;10:1111108. doi: 10.3389/fmolb.2023.1111108

FIGURE 5.

FIGURE 5

Platelet Panx1 modulates adhesion molecule gene expression in endothelial cells in vitro. (A,B) MHEC5-T cells (endothelial cell line) were incubated with platelet releasates of naïve Panx1 WT (Panx1 fl/fl -Pf4-Cre − ; n = 4), respectively Panx1 KO (Panx1 fl/fl -Pf4-Cre + ; n = 5) mice for 3 h. For platelet releasates, isolated platelets were stimulated with the indicated agonists for 15 min beforehand. After incubation with platelet releasates for 3 h, the MHEC5-T cells were harvested and gene expression of (A) P-selectin and (B) E-Selectin was analyzed via qRT-PCR. Data are normalised to WT rest. (C,D) Plasma concentration of soluble P-selectin and E-selectin of PPE operated Panx1 WT (Panx1 fl/fl -Pf4-Cre − ; n = 3) and Panx1 KO (Panx1 fl/fl -Pf4-Cre + ; n = 3) mice 14 days after surgery. Plasma samples from naïve Panx1 WT (n = 3), respectively Panx1 KO (n = 4) mice served as controls. Data are represented as mean values ±SEM. Statistical analysis was performed using (A–D) a two-way ANOVA with a Sidak’s multiple comparisons post hoc test; *p < 0.05, ***p < 0.001. CRP, Collagen-related peptide; Panx1, Pannexin-1; Rest, resting; Thr, Thrombin; PPE, Porcine Pancreatic Elastase perfusion.