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Analysis of gene expression in wild-type and mutant mouse embryos. Ten micrograms of total RNA from E11.5 embryos of the indicated genotypes was analyzed by Northern hybridization with the indicated probes. Three independent experiments yielded essentially the same results, and two representative hybridization patterns are shown here. In spite of complete lack of Six4 mRNA in Six4−/− embryos, the expression levels of the genes analyzed except Six4 were not altered.
