FIG. 5.

Expression of marker genes for somite-myotome (myogenin) and neuronal cells (neuroD3) in wild-type (A, C, and G) and Six4-deficient (B, D, and H) embryos. E11.5 (A and B) and E12.5 (C and D) embryos were analyzed for myogenin expression by in situ hybridization. Adult skeletal muscles of wild-type (E) and Six4-deficient (F) mice were also analyzed by hematoxylin-eosin staining. The expression of neuroD3, one of the neural marker genes, in E11.5 embryos was also analyzed by in situ hybridization (G and H). For both probes, the pattern and intensity of the staining were not different between wild-type and Six4-deficient embryos.