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After 2 h of TRH stimulation, F-actin and β-catenin accumulate at the cell-cell contacts. GH3B6 cells transfected with hPKCα-wt–GFP were analyzed for F-actin and β-catenin localization by immunocytochemistry using phalloidin-TRITC and an anti-β-catenin antibody. Cells were treated for 2 h with 100 nM TRH. Merged image: green, hPKCα-wt–GFP; red, either F-actin (top) or β-catenin (bottom).
