Table 2.
Antibodies used in western blot (WB) and Immunofluorescence (IF)
| Antibody/host | Company | Cat. No. | Dilution |
|---|---|---|---|
| AHR/Rabbit | Enzo Life Sciences (Farmingdale, New York) | BML-SA550-0100 | 1:1000 (WB), 1:200 (IF) |
| TUJ1/Mouse | abcam (Waltham, Massachusetts) | ab78078 | 1:1000 (WB), 1:500 (IF) |
| nNOS/Rabbit | abcam (Waltham, Massachusetts) | ab76067 | 1:2000 (WB), 1:500 (IF) |
| ChAT/Goat | MilliporeSigma (Burlington, Massachusetts) | AB144P | 1:400 (WB), 1:100 (IF) |
| Histone H3/Rabbit | Cell Signaling Technology (Danvers, Massachusetts) | 9715 | 1:2000 (WB) |
| GAPDH/Rabbit | Cell Signaling Technology (Danvers, Massachusetts) | 2118 | 1:2500 (WB) |
| HRP-conjugated anti-rabbit | Cell Signaling Technology (Danvers, Massachusetts) | 7074 | 1:2000-5000 (WB) |
| HRP-conjugated anti-mouse | Cell Signaling Technology (Danvers, Massachusetts) | 7076 | 1:2000 (WB) |
| HRP-conjugated anti-goat | R&D Systems (Minneapolis, Minnesota) | HAF017 | 1:800 (WB) |
| Donkey anti-Rabbit, Alexa Fluor 594 | Invitrogen (Waltham, Massachusetts) | A21207 | 1:200 (IF) |
| Donkey anti-Goat, Alexa Fluor 594 | Invitrogen (Waltham, Massachusetts) | A32758 | 1:200 (IF) |
| Donkey anti-Mouse, Alexa Fluor 488 | Invitrogen (Waltham, Massachusetts) | A21202 | 1:200 (IF) |