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Increased p21Waf1, MDM2, and PIG3 mRNAs after ADR and IR treatment of RKO cells. RKO cells were treated with ADR (0.4 μM) or IR (10 Gy) for the times indicated. (A and C) Cells were harvested, mRNA was isolated, and Northern analyses were performed for p21Waf1, MDM2, PIG3, and GAPDH mRNAs. (B and D) Graphic representation of the levels of mRNA in the Northern blots in panels A and C, normalized to GAPDH levels. The data are representative of two independent experiments.
