Figure 4.

AAV2.5T-binding immunoglobulin (Ig) isotype in plasma and BALF

(A–C) Plasma was harvested at the indicated time points post-rAAV2.5T-gLuc redosing for analysis of AAV2.5T-binding (A) IgG (diluted 4,000-fold for the 0-, 5-, and 10-day samples and 16,000-fold for the 14-day samples), (B) IgM (diluted to 1:2,000), and (C) IgA (diluted to 1:20). (D–F) Neat undiluted BALF was used for analysis of AAV2.5T-binding (D) IgG, (E) IgM, and (F) IgA. Results are presented as the mean ± SEM. Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Statistical significance is noted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. ns, not significant. n = 15 animals in each group.