Figure 1.

Cell types present in the hPSC-derived tissue configurations

(A) Workflow of hPSC-derived cell derivation and construction of four tissue configurations. Four tissue constructs—CS, hvCAS, hvCTS, and hvCOC—were fabricated from hPSC-CMs on day 15 post-differentiation and cultured until day 29-30 post-differentiation, followed by cell dissociation for scRNA-seq.

(B and C) UMAP plots identified seven cell types separated into distinct clusters showing cells isolated from all tissue constructs (B) and cells in each tissue construct (C).

(D) Bar plot showing the fraction of each cell type present in the four constructs.

(E) Violin plots showing expression of marker genes used to identify the seven cell types.