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. 2023 Feb 16;299(4):103036. doi: 10.1016/j.jbc.2023.103036

Figure 3.

Figure 3

Impact of aC5 supplementation on the acyl-CoA and acyl-ACP pool compositions in strain NE1896 (lpdA::φNΣ). Strains were grown in LB or LB supplemented with 1 mM aC5. A, acyl-CoA pool compositions of strains JE2 (WT) and NE1896 (lpdA::φNΣ) in the presence and absence of aC5. Acyl-CoAs were measured using LC-MS/MS with [13C2]acetyl-CoA as the internal normalization standard. B, the amounts of apo-ACP, ACPSH, and malonyl-ACP (Mal) in strains JE2 (WT) and NE1896 (lpdA::φNΣ) in the presence and absence of aC5. ACP species were measured using LC-MS/MS using [13C2]acetyl-ACP as the internal standard to calculate normalized abundance. C, the FASII cycle intermediate acyl-ACP pool composition in strains JE2 (WT) and NE1896 (lpdA::φNΣ) strains in the presence and absence of aC5. Inset, the color key for all three panels. ACP, acyl carrier protein; FASII, type II fatty acid synthase.