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. 2023 Feb 16;299(4):103036. doi: 10.1016/j.jbc.2023.103036

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© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Identification of the mbcS gene. Strains were grown in defined media lacking Ile and Leu and supplemented with 50 μM aC5. A, aC5 supplementation corrects the change in PG molecular species phenotype of strain JE2 (WT) and gives a 32:35-PG ratio of 2.5. B, the PG molecular species of strain NE1036 (mbcS::φNΣ) remained unchanged by aC5 supplementation with a 32:35-PG ratio of 0.6. C, complementation of strain NE1036 (mbcS::φNΣ) with the MbcS expression plasmid pMbcS gives a 32:35-PG ratio of 4.5. 32-PG (red); 35-PG (cyan). MbcS, methylbutyryl-CoA synthetase; PG, phosphatidylglycerol.