Figure 1.

Use of a high-resolution water-immersion objective on an inverted microscope

(A) Recording chamber configuration for bouton recordings. The red clamp attaches the pipette to the Kleindiek micromanipulator.

(B) Inverted water-immersion objective (100×, see key resources table) with Teflon sleeve to keep water in place between chamber and objective.

(C) Recording chamber with inverted water-immersion 100× objective.

(D) Custom-built pipette holder for Kleindiek micromanipulator with short electrode wire.