Reagent (MW = molecular weight in g/mol) | Final concentration | Amount |
---|---|---|
Sodium chloride (NaCl, MW = 58.3) | 150 mM | 8.74 g |
Potassium chloride (KCl, MW = 74.6) | 4 mM | 298 mg |
HEPES (MW = 238.3) | 10 mM | 2.383 g |
Glucose (MW = 180.0) | 10 mM | 1.80 g |
Magnesium chloride (MgCl2, MW = 95.2) | 1.1 mM | 105 mg |
Calcium chloride (CaCl2, MW = 111.0) | 1.1 mM | 122 mg |
ddH2O | Add for total volume of 1 L | |
Total | N/A | 1 L |